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Differentiated urothelium culture on transwell USCs were washed in PBS with 0.5 mM EDTA, trypsinized in 0.05% Trypsin and 0.5 mM EDTA for 1 min at 37 C, dissociated by vigorous pipetting, filtered through a 40 m cell strainer and resuspended in washing media

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Earlier work in Planta Medica by Liu and colleagues demonstrated that madecassoside increases collagen synthesis and reduces inflammatory infiltration in vivo [16]

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